Isolation and culture of wapiti (Cervus elaphus) satellite cells
نویسندگان
چکیده
منابع مشابه
Numbers of red deer (Cervus elaphus) and their hybrids with wapiti (Cervus elaphus spp.) farmed in New Zealand
This paper outlines findings for three experiments on the AgResearch Invermay deer farm that quantified soil and water quality via fence-line pacing and wallowing, and water quality in streams draining the farm. Five weeks after winter grazing (28 hinds/ha), fence-line pacing decreased soil macroporosity and saturated hydraulic conductivity, and increased suspended sediment (SS; 0.226 g/100ml),...
متن کاملComparison of Fatty Acids with Amino Acids Profiles of Wapiti Deer (Cervus elaphus) Muscle
The fatty acids and amino acids compositions of different parts of wapiti deer (Cervus elaphus) muscle (loin, chops, shoulder and hindquarters) were analyzed in this study. In total, fifteen types of fatty acid were identified and quantified in fresh and freeze-dried samples, with monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) constituting approximately 47.82-59.04%...
متن کاملSeasonal foraging behavioural compensation in reproductive wapiti hinds (Cervus elaphus canadensis).
Captive wapiti hinds were observed during seven periods between June 1996 and November 1997. We investigated their activity and foraging behaviour on two pastures, heavily and lightly grazed, during four phases of the reproductive cycle (early gestation, late gestation, peak lactation, and late lactation). Within season, differences in forage availability between pastures had little apparent ef...
متن کاملIsolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution
Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...
متن کاملEstimating dry matter digestibility and intake in wapiti (Cervus elaphus canadensis) using the double n-alkane ratio technique
We estimated dry matter digestibility and intake (DMI) in wapiti hinds using the double n-alkane method. We also assessed intra-ruminal controlled-release devices (CRD) for administering n-alkane markers C32 and C36. Nine wapiti hinds were offered alfalfa-based compounded diet for ad libitum consumption and actual DMI was measured daily (mean: 9.2 1.6 kg DM/day). The DMI estimate for C33:C32 (9...
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ژورنال
عنوان ژورنال: Canadian Journal of Animal Science
سال: 2000
ISSN: 0008-3984,1918-1825
DOI: 10.4141/a99-078